Comparison of a Commercial Antigen-Capture ELISA with Enrichment Culture for Detection of Salmonella from Fecal Samples
نویسندگان
چکیده
منابع مشابه
Comparison of Six Culture Methods for Salmonella Isolation from Poultry Fecal Samples
Abstract Background and Objective: Salmonellosis is one of the most important food-borne bacterial zoonotic diseases worldwide, and poultry and its products are the major sources for salmonella transmission to human. Isolation of Salmonellaenterica from poultry needs bacteriologic enrichment and selected cultures of fecal samples. In this study, different culture methods for the isolation of s...
متن کاملComparison of Dot-ELISA with microbial culture for detection of Brucella spp. in clinical specimens
Definitive diagnosis of brucellosis is made by isolation of the causative agents, which is a timeconsumingprocedure. To evaluate the efficacy of Dot-ELISA for detecting brucellae in clinical samples, 94different specimens taken from animal origin were cultured on brucella selective culture media and colonieswere identified biochemically. The specimens were also examined after centrifugation by ...
متن کاملProtein Chips for Detection of Salmonella spp. from Enrichment Culture
Food pathogens are the cause of foodborne epidemics, therefore there is a need to detect the pathogens in food productions rapidly. A pre-enrichment culture followed by selective agar plating are standard detection methods. Molecular methods such as qPCR have provided a first rapid protocol for detection of pathogens within 24 h of enrichment culture. Biosensors also may provide a rapid tool to...
متن کاملComparison of culture, ELISA and PCR techniques for salmonella detection in faecal samples for cattle, pig and poultry
BACKGROUND Performances of different salmonella detection methods were evaluated by applying them to of artificially contaminated faecal specimens from cattle, pigs and poultry. The NMKL71 method, being the standard reference method for detection of salmonella in the official Swedish control program, was compared with the proposed ISO method using MSRV-selective enrichment for culturing, and al...
متن کاملSalmonella detection in poultry samples. Comparison of two commercial real-time PCR systems with culture methods for the detection of Salmonella spp. in environmental and fecal samples of poultry.
STUDY The efficiency of two commercial PCR methods based on real-time technology, the foodproof® Salmonella detection system and the BAX® PCR Assay Salmonella system was compared to standardized culture methods (EN ISO 6579:2002 - Annex D) for the detection of Salmonella spp. in poultry samples. MATERIAL AND METHODS Four sample matrices (feed, dust, boot swabs, feces) obtained directly from p...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Veterinary Diagnostic Investigation
سال: 1994
ISSN: 1040-6387,1943-4936
DOI: 10.1177/104063879400600421